Practical Aspects of Animal Biotechnology and Immunology

by Sunita K Yadav

ISBN: 9789371703604
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Imprint : Daya Publishing House
Year : 2026
Price : Rs. 9960.00
Biblio : xv+196p., 8 col., tbls., figs., ind., 25cm

Author Profile

Dr. Sunita K. Yadav is an Assistant Professor at Daulat Ram College at the University of Delhi in India. She earned her Ph.D. from the School of Biotechnology at Jawaharlal Nehru University and graduate and post-graduate degrees from the University of Delhi. She has ten years of teaching experience, during which time she has taught courses in Genetics, Immunology, and Biotechnology. She has served as the college magazine's coordinator for two years in a row and has attended numerous National and International conferences. She has published numerous research publications in high impact journals such as fish and shellfish immunology, microbial pathogenesis, and journal of medical virology. In order to motivate students for research, she has been organizing various intercollege workshops on scientific writing and research analyses tools and techniques.

About The Book

Biotechnology and Immunology are two closely related scientific fields that have a significant impact on various aspects of human health, biomedicine, agriculture, and industry. The synergy between biotechnology and immunology is particularly evident in the development of vaccines, monoclonal antibodies, and diagnostic tools. Biotechnology plays a pivotal role in producing biopharmaceuticals and vaccines, while immunology provides the knowledge and understanding of the immune system required to design effective immunotherapies. The practical manual focusing on immunology and biotechnology is designed to serve as a comprehensive educational tool for students, specifically aligned with the undergraduate curriculum of various universities. The book's structure has been carefully crafted to simplify the intricate subject matter for learners. It encompasses detailed explanations, vibrant illustrations, and step-by-step procedures aimed at facilitating comprehension. Furthermore, the manual incorporates solved examples for experiments involving mathematical calculations. The inclusion of key terminology and end-of-chapter questions ensures that students acquire a robust understanding of the material. In order to enrich the learning experience, the book features an array of more than 60 vivid conceptual images and diagrams. The structured format of the book, coupled with its abstracts and outlined learning objectives, serves to further support students in grasping and retaining the content. Additionally, the book underscores the importance of safe laboratory practices by offering clear guidelines on what is and isn't acceptable in a laboratory setting. Ultimately, the primary objective of this book is to empower students in expanding their knowledge in the fields of biotechnology and immunology, while also assisting universities and academic institutions in developing relevant courses.

Table of Contents

Acknowledgements v Preface vii The Contributors xi Abbreviations xiii General Operating Procedures 1 Safety in Laboratories 3 Part-I 1. Isolation of Genomic DNA from E. coli 7 2. Isolation of Plasmid DNA (pUC 18/19) from E. coli 17 3. To Perform Restriction Digestion of Plasmid DNA 27 4. Preparation of Competent Cells and Transformation of the Cells Using pUC18 to Calculate the Transformation Efficiency 33 5. Construction of Circular and Linear Restriction Maps from the Data Provided 43 6. To Study the Technique of DNA Fingerprinting 51 7. Study of Polymerase Chain Reaction 61 8. Western Blotting 71 9. To Demonstrate the Technique of Southern Hybridization 85 10. Northern Blotting 91 Part-II 1. Demonstration of Lymphoid Organs in Rat 99 2. Histological Study of Spleen, Thymus and Lymph Nodes through Permanent Slides/Micrographs 111 3. Preparation of a Stained Blood Film to Study Types of Cells 125 4. Preparation of Single Cell Suspension of Splenocytes, Cell Counting and Viability Test 139 5. To Determine the ABO Grouping of the Blood Sample 147 6. Detection of Complement Activity Using Hemolysis of Antibody Coated SRBC and Standard Serum 155 7. Enzyme-Linked Immunosorbent Assay (ELISA) 163 8. To Learn the Technique and Basic Patterns of Precipitation by Ouchterlony’s Double Immuno-diffusion Method 173 9. To Learn the Technique of Immuno-electrophoresis 181 Glossary 189 Colour Plates I-VIII Index 195