Experimental Biotechnology: Practical Manual Series
by Sunita Dutta & Abhijit Dutta
ISBN: 9789351244660
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Imprint : NIPA
Year : 2021
Price : Rs. 7505.00
Biblio : 268 pages,25cm
Author Profile
Sunita Dutta: Reader, Department of Zoology, Women’s College, Ranchi,Jharkhand. Abhijit Dutta: Reader, Department of Zoology, Ashok Kumar Choudhary: Professor, Department of Botany University of Ranchi (Jharkhand)
About The Book
The book is subdivided into seven sections this encompass: general procedures, like methods of pipetting, solution preparation, buffers and principles of common analytical instruments essential for laboratory biotechnology experiments. The book also includes working with nucleic acid, bacteria, enzymes, proteins; cloning experiments and a few protocols on plant biotechnology. Emphasis have been given on DNA/RNA isolation from various sources, use of restriction enzymes, ligation techniques, cloning protocols, screening of transformed cells, various electrophoresis techniques, PCR protocol, etc. The appendices in the last part are included to provide information important to he study of the above-mentioned practical as a whole. The book will be useful to students belonging to Biotechnology, agriculture and allied fields. The idea behind this practical manual was thus to provide theoretical basis of the practical study items to be undertaken in the laboratory in a lucid manner.
Table of Contents
"Preface
Abbreviation
Section-1 : General Procedures
Chapter-1 : Introduction to the Laboratory
Chapter-2 : Pipetting
Chapter-3 : Measuring Absorbance
Chapter-4 : Preparing Dilutions
Chapter-5 : Laboratory Buffers
Chapter-6 : Operating Systems of Common Instruments
Section-2 : Nucleic Acids and E.coli
Chapter-7 : Working with Nucleic Acids
Chapter-8 : Working with E.coli
Chapter-9 : Plymerase Chain Reaction
Chapter-10 : Plasmid Preparation
Chapter-11 : Plasmid DNA Isolation from E.coli
Chapter-12 : Plasmid Miniprep
Chapter-13 : Plasmid Midi-Prep from Bacteria
Chapter-14 : DNA Plasmid Maxiprep
Chapter-15 : Isolation of Genomic DNA from Bacteria
Chapter-16 : DNA Preparation from Cell Lines, High Salt Method
Chapter-17 : DNA Preparation from Fresh Frozen Tissues
Chapter-18 : DNA Preparation from Paraffin Tissue.
Section-3 : Enzymes
Chapter-19 : Restriction Digestion
Chapter-20 : Restriction Digestion of ?-DNA with Hind III or Eco RI
Chapter-21 : Digestion with a Single and Double Restriction Enzyme
Chapter-22 : Quick Restriction Digestion
Chapter-23 : Ligation Reaction
Chapter-24 : Fast Ligation of DNA
Chapter-25 : DNA Ligation with T4 DNA Ligase
Section-4 : Cloning
Chapter-26 : Competent Cell Preparation
Chapter-27 : Cloning via Restriction Digests
Chapter-28 : Transformation of Competent Cells
Chapter-29 : Selection and Screening of Transformed Cells
Section-5 : DNA/RNA Separation
Chapter-30 : Electrophoresis
Chapter-31 : Agarose Gel Electrophoresis
Chapter-32 : Extraction of DNA from Gel
Chapter-33 : Electroelution of DNA from Gel into Dialysis Tubing
Chapter-34 : RNA Gel Electrophoresis
Chapter-35 : Polyacrylamide Gel Electrophoresis (PAGE)
Chapter-36 : Polyacrylamide Gel Electrophoresis of RNA
Chapter-37 : Silver Nitrate Staining
Chapter-38 : DNA Sequencing
Section-6 : Proteins
Chapter-39 : SDS PAGE for Protein Separation
Chapter-40 : Cell Lysis for Protein Purification
Chapter-41 : Western Blot Analysis of Proteins
Section-7 : Plant Biotechnology
Chapter-42 : Plant Tissue Culture Techniques
Chapter-43 : In Vitro Germination of Orchid Seeds
Chapter-44 : Micropropagation-Initiation of Miniature Rose Shoot Cultures
Chapter-45 : Potato Transformation Protocol
Chapter 46 : Transformation : Agrobacterium (Freeze Thaw Method)
Appendices
Appendix-1 : Solutions & Buffers
Appendix-2 : Primers
Appendix-3 : Units & Formulas
Appendix-4 : DNA Related Information
Appendix-5 : Amino Acids
Glossary
References
Index"