Experimental Biotechnology: Practical Manual Series

by Sunita Dutta & Abhijit Dutta

ISBN: 9789351244660
View Ebook
Imprint : NIPA
Year : 2021
Price : Rs. 7505.00
Biblio : 268 pages,25cm

Author Profile

Sunita Dutta: Reader, Department of Zoology, Women’s College, Ranchi,Jharkhand. Abhijit Dutta: Reader, Department of Zoology, Ashok Kumar Choudhary: Professor, Department of Botany University of Ranchi (Jharkhand)

About The Book

The book is subdivided into seven sections this encompass: general procedures, like methods of pipetting, solution preparation, buffers and principles of common analytical instruments essential for laboratory biotechnology experiments. The book also includes working with nucleic acid, bacteria, enzymes, proteins; cloning experiments and a few protocols on plant biotechnology. Emphasis have been given on DNA/RNA isolation from various sources, use of restriction enzymes, ligation techniques, cloning protocols, screening of transformed cells, various electrophoresis techniques, PCR protocol, etc. The appendices in the last part are included to provide information important to he study of the above-mentioned practical as a whole. The book will be useful to students belonging to Biotechnology, agriculture and allied fields. The idea behind this practical manual was thus to provide theoretical basis of the practical study items to be undertaken in the laboratory in a lucid manner.

Table of Contents

"Preface Abbreviation Section-1 : General Procedures Chapter-1 : Introduction to the Laboratory Chapter-2 : Pipetting Chapter-3 : Measuring Absorbance Chapter-4 : Preparing Dilutions Chapter-5 : Laboratory Buffers Chapter-6 : Operating Systems of Common Instruments Section-2 : Nucleic Acids and E.coli Chapter-7 : Working with Nucleic Acids Chapter-8 : Working with E.coli Chapter-9 : Plymerase Chain Reaction Chapter-10 : Plasmid Preparation Chapter-11 : Plasmid DNA Isolation from E.coli Chapter-12 : Plasmid Miniprep Chapter-13 : Plasmid Midi-Prep from Bacteria Chapter-14 : DNA Plasmid Maxiprep Chapter-15 : Isolation of Genomic DNA from Bacteria Chapter-16 : DNA Preparation from Cell Lines, High Salt Method Chapter-17 : DNA Preparation from Fresh Frozen Tissues Chapter-18 : DNA Preparation from Paraffin Tissue. Section-3 : Enzymes Chapter-19 : Restriction Digestion Chapter-20 : Restriction Digestion of ?-DNA with Hind III or Eco RI Chapter-21 : Digestion with a Single and Double Restriction Enzyme Chapter-22 : Quick Restriction Digestion Chapter-23 : Ligation Reaction Chapter-24 : Fast Ligation of DNA Chapter-25 : DNA Ligation with T4 DNA Ligase Section-4 : Cloning Chapter-26 : Competent Cell Preparation Chapter-27 : Cloning via Restriction Digests Chapter-28 : Transformation of Competent Cells Chapter-29 : Selection and Screening of Transformed Cells Section-5 : DNA/RNA Separation Chapter-30 : Electrophoresis Chapter-31 : Agarose Gel Electrophoresis Chapter-32 : Extraction of DNA from Gel Chapter-33 : Electroelution of DNA from Gel into Dialysis Tubing Chapter-34 : RNA Gel Electrophoresis Chapter-35 : Polyacrylamide Gel Electrophoresis (PAGE) Chapter-36 : Polyacrylamide Gel Electrophoresis of RNA Chapter-37 : Silver Nitrate Staining Chapter-38 : DNA Sequencing Section-6 : Proteins Chapter-39 : SDS PAGE for Protein Separation Chapter-40 : Cell Lysis for Protein Purification Chapter-41 : Western Blot Analysis of Proteins Section-7 : Plant Biotechnology Chapter-42 : Plant Tissue Culture Techniques Chapter-43 : In Vitro Germination of Orchid Seeds Chapter-44 : Micropropagation-Initiation of Miniature Rose Shoot Cultures Chapter-45 : Potato Transformation Protocol Chapter 46 : Transformation : Agrobacterium (Freeze Thaw Method) Appendices Appendix-1 : Solutions & Buffers Appendix-2 : Primers Appendix-3 : Units & Formulas Appendix-4 : DNA Related Information Appendix-5 : Amino Acids Glossary References Index"